31 มี.ค. 3109

Tropical Life Sciences Research

34

85-98

Penerbit Universiti Sains Malaysia

The objective of this study was to investigate the mixed culture of Bacillus subtilis, B. licheniformis, and B. megaterium to control acute hepatopancreatic necrosis disease (AHPND) or EMS (Early Mortality Syndrome) in white shrimp Litopenaeus vannamei as a model. The infected shrimps with Vibrio parahaemolyticus AHPND strain were divided into tanks and different feeding with either B. subtilis, B. licheniformis, or B. megaterium and feeding with the mixed culture of all Bacillus strains. The infected shrimps were fed with the mixed culture of Bacillus that showed the significant highest survival rate and revealed lower percent detection of V. parahaemolyticus AHPND strain by Polymerase Chain Reaction (PCR) (57.14%) with a small amount of viability count in their hepatopancreas. In contrast, the infected shrimp feeding with each of B. subtilis, B. licheniformis, or Bacillus megaterium, revealed the spread of V. parahaemolyticus AHPND strain in all tissue by PCR detection (86.67-100%) with a large amount of viability count (3.53-4.24x103 CFU/g). This study indicated that the mixed culture of Bacillus subtilis, B. licheniformis, and B. megaterium be able to control the dissemination of V. parahaemolyticus in shrimps, especially in hepatopancreatic that is the target tissue of AHPND in white shrimp (L. vannamei). The result of this study revealed the efficiency and mechanism of the mixed culture 3 of Bacillus subtilis, B. licheniformis, and B. megaterium to control the virulence of AHPND and support the application of this mixed culture in aquaculture of shrimp farms to avoid chemical and antibiotic treatment by using it as a biological control.

https://ejournal.usm.my/tlsr/article/view/tlsr_vol34-no-1-2023_6/pdf