Decreased Severity of Acute Hepatopancreatic Necrosis Disease in White Shrimp (Litopenaeus vannamei) by Mixed Culture of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium
31 มี.ค. 3109
Tropical Life Sciences Research
Penerbit Universiti Sains Malaysia
The objective of this study was to investigate the mixed culture
of Bacillus subtilis, B. licheniformis, and B. megaterium to control acute
hepatopancreatic necrosis disease (AHPND) or EMS (Early Mortality Syndrome)
in white shrimp Litopenaeus vannamei as a model. The infected shrimps
with Vibrio parahaemolyticus AHPND strain were divided into tanks and different
feeding with either B. subtilis, B. licheniformis, or B. megaterium and feeding with
the mixed culture of all Bacillus strains. The infected shrimps were fed with the
mixed culture of Bacillus that showed the significant highest survival rate and
revealed lower percent detection of V. parahaemolyticus AHPND strain by
Polymerase Chain Reaction (PCR) (57.14%) with a small amount of viability count
in their hepatopancreas. In contrast, the infected shrimp feeding with each of B.
subtilis, B. licheniformis, or Bacillus megaterium, revealed the spread of V.
parahaemolyticus AHPND strain in all tissue by PCR detection (86.67-100%) with
a large amount of viability count (3.53-4.24x103 CFU/g). This study indicated that
the mixed culture of Bacillus subtilis, B. licheniformis, and B. megaterium be able
to control the dissemination of V. parahaemolyticus in shrimps, especially in
hepatopancreatic that is the target tissue of AHPND in white shrimp (L. vannamei).
The result of this study revealed the efficiency and mechanism of the mixed culture
of Bacillus subtilis, B. licheniformis, and B. megaterium to control the virulence of
AHPND and support the application of this mixed culture in aquaculture of shrimp
farms to avoid chemical and antibiotic treatment by using it as a biological control.