Anti-Inflammatory Activity of Geldanamycin and Its Derivatives in LPS-Induced RAW 264.7 Cells
ผู้เขียน :
Thongchai Taechowisan
,
Winyou Puckdee
,
Watcharee Waratchareeyakul
,
Waya S. Phutdhawong
เผยแพร่วันที่ :
29 เม.ย. 2562
ประเภท :
บทความวิจัยที่ตีพิมพ์ในวารสารวิชาการระดับนานาชาติ
วารสารวิชาการ :
Advances in Microbiology
เล่มที่ :
9
ฉบับที่ :
4
หน้า :
398-421
ผู้เผยแพร่ :
Scientific Research Publishing
รายละเอียด :
Geldanamycin (1) had been isolated as a major compound from Streptomyces zerumbet W14; an endophyte of Zingiber zerumbet (L.) Smith. Two new geldanamycin derivatives; 17-(tryptamine)-17-demethoxygeldanamycin (2) and 17-(5’-methoxytryptamine)-17-demethoxygeldanamycin (3) were synthe-sized and their anti-inflammatory activity was evaluated in LPS-induced macrophage RAW 264.7 cells by investigating their effects on the inhibition of production of NO, PGE2, TNF-α, IL-1β, IL-6 and IL-10. The data obtained were consistent with the modulation of TNF-α, IL-1β, IL-6, IL-10 production by these derivatives at concentration of 1 to 5 µg/ml. A similar effect was also observed when LPS-induced NO release and PGE2 production were tested. The inhibitory effects were shown in concentration-dependent manners. From the obtained results, it was concluded that two new geldanamycin derivatives possess anti-inflammatory activity on LPS-induced RAW 264.7 cells. They could be useful for the management of inflammatory diseases. [Keywords : Anti-Inflammatory Activity, Geldanamycin Derivatives, Streptomyces zerumbet, RAW 264.7 Cells]
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